沙土鼠脑缺血时微血管一氧化氮的释放规律及机制

Mechanism and rule of nitric oxide release from cerebral microvessel in gerbils with cerebral ischemia

目的 测量脑缺血时沙土鼠软脑膜细动脉中一氧化氮 (NO)含量的变化及胆碱能受体阻断后的效应。方法 采用电化学方法。结果 脑缺血时 ,细动脉内的NO合成增加 ,呈周期间断性释放 ,周期间隔为 5～ 8min ,持续时间 3～ 5min。软脑膜表面滴入阿托品后 ,NO呈周期间断性释放现象消失 ,所测得的NO仍明显增加。结论 脑缺血时 ,乙酰胆碱分泌可能增加 。

Objective To investigate the changes of NO as well as the effect of blocked choline receptor in pia mater microarteries in gerbils with cerebral ischemia. Methods A total of 16 gerbils were inflicted with bilateral common carotid arteries and divided into ischemic group and ischemia+atropine, the other 8 animals served as control. The changes of NO were measured with electrical chemistry. Results During cerebral ischemia, the NO was increased in pia mater microartery and released periodically for 3～5 min in an interval of 5～8 min and continued. When atropine (0.1mmol/L) was dropped onto the surface of gerbil's brain, the periodical release of NO disappeared, but the NO was still higher in pia mater microarteries than basic content. Conclusion The NO release from cerebral microvessel may be partly regulated by acetycholine during cerebral ischemia.