美兰对变形链球菌代谢的作用

The effect of methylene blue on the metabolism of Streptococcus mutans

目的 :研究美兰对变形链球菌活性氧代谢及产酸代谢的作用 ,探讨美兰防龋的可行性。方法 :采用电子自旋捕捉法研究美兰对变形链球菌活性氧代谢的作用 ;采用气相色谱法研究美兰对变形链球菌产酸的抑制作用。结果 :美兰使变形链球菌糖代谢过程中产生的特征性的DMPO -O-·2 和DMPO -OH·ESR信号消失 ,并使得变形链球菌产酸总量显著减少。结论 :美兰对变形链球菌活性氧代谢和产酸代谢具有明显的抑制作用 ;

Objective: To study the effect of methylene blue on the metabolism of reactive oxygen and acid production of Streptococcus mutans . Methods: Streptococcus of cricetusa, rattusb, mutansc, sobrinsd and mutans e,f and g were exposed to methylene blue at 2.5×10 -4 g/L for 1 h. The metabolism of reactive oxygen and acid production were detected by electron spin trapping method and gas chromatography respectively. Results: Electron spin resonance (ESR) spectrums of DMPO-O - · 2 and DMPO-OH · were observed in Streptococcus mutans cultured in glucose fluid, but were not found in those exposed to methylene. The total of acid production by Streptococcus mutans incubated with glucose + methylene blue were significantly lower than that with single glucose. Conclusion: Methylene blue may inhibit oxygen species metabolism and acid production of Streptococcus mutans , it may be used as a kind of dental caries prevention agent.