亚胺培南在烧伤早期家兔痂下组织液的药动学实验研究

An experimental study on the pharmacokinetic of Imipenem in the interstitial fluids under eschar of burn rabbits in the early stage

目的 研究亚胺培南在严重烧伤早期家兔痂下组织液中的药代动力学变化。方法 烧伤组 :烧伤早期家兔 ;对照组 :正常非烧伤家兔于 2 0min内iv10 0mg泰能后 ,用微生物琼脂打孔扩散法测定痂下组织液和血浆中亚胺培南的浓度 ,利用 3P87软件计算其相关药代动力学参数 ,采用SPSS8.0软件包进行统计学处理。结果 与对照组相比 ,烧伤组亚胺培南药代动力学参数发生显著改变。烧伤组血浆和痂下组织液中亚胺培南T1/ 2 明显延长 ,分别为血浆 (1.4 9± 0 .2 1)h、痂下组织液 (2 .4 9± 0 .4 3)h ;对照组血浆 (1.10± 0 .18)h ;亚胺培南在痂下组织液中在用药后 1h即可以检测到 ,有效浓度可维持 6～ 8h。结论 亚胺培南在烧伤早期痂下组织液和血浆中T1/ 2 明显延长 ,其在痂下组织液中形成抗生素保护屏障 。

Aim To study the changes of pharmacokinetic parameters of Imipenem in the interstitial fluids under eschar of burn rabbits in the early stage.Methods Concentrations of Imipenem in the interstitial fluids under eschar and plasma of 8 burn rabbits and those in plasma of 8 control rabbits were determined by microbial agar diffusion assay after the first initial dose of 100 mg of Imipenem/Cilastatin in 20 minutes,pharmacokinetic parameters of Imipenem were produced by program 3P87 and statistically analyzed by program package SPSS 8.0.Results Compared to those in control group,pharmacokinetic parameters of Imipenem exhibited evident difference.Half-life prolonged,(1.49±0.21)h in plasma and (2.49±0.43)h in the interstitial fluids under eschar of burn group,(1.10±0.18)h in plasma of control group.Imipenem could be detected in the interstitial fluids under eschar of burn rabbits in 1h after the first initial dose of 100mg.The effective concentrations of Imipenem could maintain 6～8h.Conclusion Half-life of Imipenem prolonged in the interstitial fluids under eschar and plasma of burn rabbits in the early stage.The antibiotic barrier could form in the interstitial fluids under eschar,and could prevent an invasive bacterial infection from burn wound.