黄芪注射液对大鼠睡眠剥夺后脑组织NO含量和SOD活性的影响

The effect of Radix Axtragali injection on levels of superoxide dismutase and nitric oxide in rat brain after sleep deprivation

目的 观察黄芪注射液对大鼠完全睡眠剥夺 (TSD)后脑组织一氧化氮 (NO)含量和超氧化物歧化酶 (SOD)活性的影响及行为变化 ,探讨黄芪对睡眠剥夺的保护作用。方法 采用小平台水环境法制作大鼠TSD模型 ,观察大鼠经过 3dTSD后额叶和海马NO含量和SOD活性和行为变化 ,并观察黄芪干预对这些指标的影响。结果 与正常对照组比较 ,TSD大鼠额叶和海马NO含量和SOD活性均升高 ,行为改变明显。黄芪干预后大鼠脑内NO含量及SOD活性明显下降 (P <0 .0 1～ 0 .0 5 )并能减轻其行为改变。结论 黄芪注射液具有改善生化代谢作用 。

Aim To observe the content of nitric oxide (NO) and the activity of superoxide dismutase (SOD) in rat brain and behavior Changes after total sleep deprivation (TSD) and to explore the effect of Radix Axtragali on TSD.Methods NO levels and SOD activity in the front cortex and hippocampus of Sprague-Dawley rats were assayed after TSD for 3d induced by 'flower pot' technique.Results NO levels and SOD activity in the front cortex and hippocampus after TSD were increased compared with those of the control cage groups (CC)(P<0.01) and significantly decreased compared with those of the groups of Radix Axtragali(P<0.01～0.05).There were great behavior changes after TSD and the Radix Axtragali could relieve those changes.Conclusion The results indicate that NO and Oxygen free radical may participated in the brain damage mechanisms during sleep deprivation and Radix Axtragali can reduce the effect.