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RNA干涉分子机制研究进展 被引量：15

Progress in Molecular Mechanism of RNA Interference
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摘要 RNA干涉 (RNAinterference ,RNAi)是生物体内的一种通过双链RNA (dsRNA)来抵抗病毒入侵和抑制转座子活动的自然机制 .双链RNA与同源mRNA互补结合而使特定基因失活 ,这一过程已经在包括拟南芥、线虫和真菌等多种模式生物中得到揭示 .近来研究表明 ,2 1～ 2 5nt的小干涉RNA (smallinterferenceRNA ,siRNA)可介导哺乳动物细胞特异性基因沉默 .RNAi具有高效性和高度特异性 。 RNA interference (RNAi) is a natural mechanism in organisms in resistance to virus invasion and inhibition of transposon mobility by double-stranded RNA (dsRNA). dsRNA can match with homologous mRNA by base paring to make specific gene inactivation. RNAi was observed in many model organisms, such as Arabidopsis, C. elegans and fungi. Latest study shows that 21 similar to 25 nt small interference RNA (siRNA) can mediate specific gene silencing in mammal cells. Being effective and highly specific, RNAi probably becomes a novel technique in knocking gene down and plays important roles in gene function study and gene therapy of diseases.
作者 孙建国 廖荣霞 陈正堂
机构地区 第三军医大学附属新桥医院全军肿瘤中心 第三军医大学基础部生物化学教研室
出处 《生物化学与生物物理进展》 SCIE CAS CSCD 北大核心 2002年第5期678-681,共4页 Progress In Biochemistry and Biophysics
关键词 RNA干涉 分子机制 研究进展 基因沉默 RNA interference small interference RNA gene silencing
分类号 Q522 [生物学—生物化学] Q75 [生物学—分子生物学]
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  • 1Fire A,Xu S,Montgomery M K,et al. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature,1998,391(6669):806～811
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  • 3Elbashir S M,Harborth J,Lendeckel W,et al. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature,2001,411(6836):494～498
  • 4Ravi S K,Maruxa M C,Peder Z,et al. Effectiveness of specific RNA-mediated interference through ingested double-stranded RNA in Caenorhabditis elegans. Genome Biol,2001,2(1):RESEARCH0002.1～0002.10
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生物化学与生物物理进展
2002年 第5期

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