摘要
利用转抗白叶枯病基因Xa2 1的水稻材料 ,通过TAIL PCR方法扩增T DNA整合的侧翼序列。从中筛选属于水稻基因组DNA的T DNA整合的侧翼序列作为探针 ,将外源基因整合位点定位到窄叶青 /京系 17DH群体构建的水稻分子连锁图谱上。共获得属于水稻基因组DNA的T DNA侧翼序列 2 2个 ,其中的 19个序列在定位群体的两个亲本之间显示RFLP多态性 ,分别定位在水稻基因组的第 3,4 ,5 ,7,9,10 ,11和 12染色体上。带有转基因Xa2 1的T DNA整合的定位为研究外源基因在不同染色体位点的位置效应和稳定遗传打下基础。
The transformation mediated by Agrobacterium has been successfully applied to rice in recent years. In the previous research we have transferred the Xa21 gene into five rice varieties of China, using Agrobacterium mediated trasformation. In this study, T DNA flanking sequences of Xa21 transgenic rice lines were obtained by using thermal asymmetric interlaced PCR (TAIL PCR). The flanking sequences which are actual rice DNA were identified and located on molecular linkage map developed from a ZYQ8/JX17 double haploid (DH) population. A total of 22 T DNA flanking rice sequences were isolated. Nineteen of them displayed RFLPs between the two parents, ZYQ8 and JX17, and were mapped on the rice chromosomes, 3, 4, 7, 9, 10, 11 and 12, respectively. The genetic mapping of T DNA integration sites in Xa21 transgenic rice will benefit the study of position effect and stable inheritance of the transgene Xa21.
基金
国家 8 63高技术项目 (No .10 1 0 1 0 2 0 1)
国家转基因植物专项 (No .J99 B 0 0 6)资助~~
