亚麻抗锈病基因M4的特异分子标记

Specific Molecular Markers of the Rust Resistance Gene M4 in Flax

用 5 2 0个 10碱基随机引物对含有亚麻抗锈病基因M4的近等基因系材料NM4及其轮回亲本Bison进行RAPD分析 ,其中OPA18引物在NM4材料中稳定地扩增出特异的DNA片段。用Bison与NM4杂交产生的F2 分离群体进行的遗传连锁性分析表明 ,RAPD标记OPA1843 2 与M4基因紧密连锁 ,二者之间的遗传距离为 2 .1cM。将OPA1843 2 片段回收、克隆和测序 ,成功地将其转化为SCAR标记。对不同抗源材料的扩增分析表明 ,该标记是M4基因的特异标记。目前这一标记已成功地应用于亚麻抗锈病基因M4的分子标记辅助选择育种。

Flax ( Linum usitatissimum L.) is an important fiber and oil producing crop. Flax rust, caused by Melampsora lini Ehrenb. Lev.,occurs worldwide and can cause severe losses in seed yield and fiber quality. In order to identify molecular markers linked to the flax rust resistant gene M4 ,RAPD analysis of NM4, a near isogenic line containing the M4 gene,and the recurrent parent Bison was carried out with 540 decamer primers. The primer OPA18 could stably amplify a specific fragment,OPA18 432 ,in the NM4 line. The OPA18 432 marker was testified to be closely linked to the M4 gene with a genetic distance of 2.1cM through the analysis of the F 2 mapping population derived from a cross of Bison×NM4. Based on the sequence of OPA18 432 , the specific PCR primers were designed,and a SCAR marker for the M4 gene was produced. Amplification of different resistant materials proved that the maker is specific for the M4 gene. This marker has been used successfully in marker assisted selection in the flax breeding program.