鸟苷发酵过程代谢流迁移的分析

Analysis on Metabolic Flux Shift During Guanosine Fermentation

以典型的代谢控制发酵产品鸟苷为例说明了一种基于过程参数的相关分析来研究发酵过程中代谢流迁移的方法。通过对发酵过程多参数的相关性分析 ,结合生物合成代谢途径、氨基酸和有机酸积累的分析 ,确认了发酵过程代谢流向EMP途径的迁移 ,认为造成这种代谢流迁移的原因可能是过程铵离子积累。在此基础上 ,通过对过程参数实时检测分析和及时调整EMP和HMP代谢通量使产率提高了 35 %。

Taking the typical metabolic control product-guanosine as an example, the method of metabolic flux shift investigation based on process multi-levels parameter correlation analysis was established. The metabolic pathway, multi-parameter correlation, accumulation of amino acid and organic acid during guanosine fermentation process were integratively analyzed. The metabolic flux shift from HMP to EMP was ascertained, which was assumed to be caused by the accumulation of ammonium ion. The subsequent optimization based on controlling flux distribution between EMP and HMP did improve the yield by 35% when the metabolic flux shift was prevented.