转基因(SAG12-IPT)青菜的迟衰特性

Senescence Delay Characterization of Transgenic Brassica chinensis L. Containing an Anti-senescence Chimeric Gene SAG12-IPT

利用根癌农杆菌感染方法将融合基因SAG12 IPT导入青菜 ,转基因植株明显表现出衰老延迟的生理现象。SAG12 IPT的抗衰老作用表现为 :在衰老过程中转基因青菜叶片中叶绿素含量高于未转基因的青菜 ,PCR分析结果表明该融合基因已经转入青菜中。激素检测结果表明转基因青菜叶片中细胞分裂素含量高于未转基因植株 ,说明抗衰老与叶片内细胞分裂素含量提高有关。另外 ,转基因植株不仅表现出活体植株衰老延迟 ,而且长在植株上的与离体的叶片滞绿时间延长。这些为蔬菜的耐储存育种提供了新的思路 ,同时为该融合基因在十字花科经济作物中的应用提供了理论依据。

An anti-senescence chimeric gene SAG12-IPT was introduced into B. chinensis L. via Agrobacterium tumefaciens-mediated transformation. PCR analysis demonstrated that the SAG12-IPT had been integrated into B. chinensis L.(Fig.2).The physiological analysis showed that senescence in transgenic plants were markedly delayed, with higher chlorophyll content than that in the wild-type during plant senescence (Figs.3 and 4 ). In addition, cytokinin content in leaves of transgenic plants was much higher than that in leaves of the wild-type plants (Fig.5). Our results showed that the SAG12-IPT transformation provides a potentially useful means in breeding of the vegetable with a higher vegetative productivity and durability of storage.