摘要
本试验对转基因植物检测中的DNA提取和PCR扩增程序作了改进。经试验 ,本研究建立的DNA快速提取法与目前广泛使用的CTAB法相比更为简便、快速和经济 ,提取的DNA质量及扩增效果无明显差异 ,可用于多种转基因植物、多种植物组织的DNA提取。利用复合PCR法可在同一反应管中同步检测 35S、NOS及CP4-EPSPS基因 ,明显提高了检测效率。应用本试验建立的DNA快速提取 -复合PCR扩增 -银染检测技术可在 6小时内得出结果 ,达到了快速、简便、灵敏、可靠的检测目的。
A rapid method for DNA extraction developed in this study has no difference in detection result from CTAB method that is widely used in genetically modified plant detection, but is more simple, quick and economic. Using multiplex PCR, 35S, NOS and CP4-EPSPS transgenes can be detected in one reaction tube at same time. A rapid, efficient and sensitive detection procedure was developed by combining rapid DNA extraction method, multiplex PCR and silver staining of DNA in polyacrylamide.
出处
《生物技术通报》
CAS
CSCD
2002年第4期39-42,46,共5页
Biotechnology Bulletin
