基因重组HIV-1 P24抗原的应用分析

Analysis of Recombinant HTV-1 P24 Antigen

目的 分析重组HIV-1-p24抗原,以便研制HIV-抗体诊断试剂。方法 以纯化重组P24为包被抗原,应用ELISA检测HIV-Ab国家参比品和正常人血清。结果20份阳性HIV-Ab检出阳性19份,符合率为95％;20份阴性HIV-Ab检出阴性18份,符合率为90％;50份正常人血清检测结果均为阴性。结论 所研制的重组P24抗原具有较高的特异性和敏感性,可用于制备HIV抗体诊断试剂。

Objective To analyze the recombinant HIV-1 p24 antigen prepared by the authors and lay a foundation of developing diagnostic kit for HIV antibody. Methods National reference for HIV-Ab and normal human sera were detected by ELISA using recombinant p24 as coated antigen. Results The accuracy rates of recombinant p24 antigens used for detecting HIV-Ab positive and negative references were 95% (19/20) and 90%(18/20) respectively. However,no HIV-Ab was detected in 50 specimens of normal human sera. Conclusion The prepared recombinant p24 antigen showed high specificity and sensitivity and was suitable for developing diagnostic kit for HIV-Ab.