利用向日葵野生种创建耐菌核病群体和自交系选育的研究

Study on Creating the Population with Tolerance to Sclerotinia by Sunflower Wild Species and Selecting Inbred Line

以向日葵优良群体为母本 ,具有耐菌核病基因的向日葵野生种为父本进行杂交 ,利用幼胚培养技术对远缘杂交后代进行“胚拯救” ,从而获得具有耐菌核病 (SclerotiniaSclerotiorumLib)基因的 10个特性群体。在这些群体中 ,葵盘菌丝体接种感病点直径平均值 (cm )比对照减少6 9 5 6 % ,根部菌核接种发病株率比对照减少 92 95 %。在耐菌核病群体中 ,运用轮回选择结合抗菌核病鉴定 ,选育出了

The crossing have been done by excellent sunflower population which used for female lines and sunflower wild species with tolerance genes to Sclerotinia Sclerotiorum Lib used for male lines.The progeny of hybridization are 'embryo rescue'with embryo culture technique.10 populations with tolerance to Sclerotinia Sclerotiorum Lib have been obtained.In these populations,compared with the check,the susceptible diameter decrease by 69.56%when inoculating on the disk.The susceptible rate decrease by 92.95% when inoculating in the root.In these tolerance populations,8 inbred lines with high tolerance to Sclerotinia Sclerotiorum Lib are selected by the combination method of recurrent selection and resistant identification.