尾分析法检测肉鸡肥度性状关联的RAPD标记

DETECTION OF THE LINKAGE BETWEEN RAPD MARKERS AND BROILER FATNESS TRAITS BY TAIL ANALYSIS METHOD

以肉仔鸡为试验对象 ,按公、母分群饲养 ,分别测定 49日龄体重、腹脂重、腹脂率和血浆 VLDL浓度 ,并对各性状表型值分为高、低两组 ,提取每组单个个体及其混合血样 DNA后 ,对核 DNA模板进行 PCR扩增。结果显示 :1各个体 DNA扩增条带在混合 DNA扩增条带中均能出现 ,表明用混合 DNA可替代单个 DNA进行群体遗传结构分析 ;2比较各性状表型值高、低组的混合 DNA扩增片段后发现 ,公、母鸡群分别有 1 2、8个 RAPD标记与性状关联。结果表明 :在数量性状位点标记检测时 ,用尾分析 (选择 DNA池 )方法可明显减少基因型检测次数 。

The 36 female and 36 male broilers were studied. At the age of 49 days, abdominal fat weight, percentage of abdominal fat and plasma VLDL concentration were measured. High and low group were divided in male or female population according to individual traits, DNA isolated from mixture blood in each group was used to amplify with PCR technique. The results were as follows: ① All bands amplified with individual DNA was found in mixed DNA, which showed mixed DNA could be used to analyze the genetic structure of population in place of individual DNA. ② Compared with mixed DNA amplified bands of high and low group of each trait, there were 12 or 8 RAPD marker traits association in male or female population respectively. The results indicated that tail (selective DNA pooling) analysis method could provide a substantial reduction in the total amount of genotypes required for marker QTL linkage determination and had better power.