肺癌中染色体的原位缺口移位技术

In Situ Nick Translation Technique of Metapnase Chromosomes in Human Lung Cancer

本文对肺腺癌中BrdU掺入染色体原位缺口移位B显带方法进行了研究。将BrdU掺入肺腺癌细胞中96小时后收获制片,老化7天后对染色体标本进行预处理及原位缺口移位。在经预处理后的肺腺癌中期染色体上获得了良好的原位缺口移位B带带型。并发现肺腺癌染色体原位缺口移位最佳DNase-1浓度为2.0ng/ml,最佳反应温度为15℃,最佳反应时间为90分钟。

A method of in situ nick translated chromosomes which were completely substituded with BrdU in h(?)nan lung adenocarcinoma cell lines was presented. Cancer cells grow in RPMI 1640 medium supplemented with FCS and BrdU (30μg/ml) at 37℃ for 96 hours. Fixed mitotic cells were preparated. In situ nick translated chromosomes were stained with 0.1% basic fuchsin and show fine nick translated bands. In this test, we found that optimal temperature, time and DNase-Ⅰ concentration for in situ nick translation of metaphase chromosomes substituted with BrdU in lung cancer were 15℃, 90 minutes and 2ng/ml.