镉对雄性大鼠垂体促性腺细胞影响的电镜形态计量学及血浆FSH含量测定研究

STUDIES ON THE EFFECT OF CADMIUM ON ULTRASTRUCTURAL MORPHOMETRY OF GONADOTROPHS AND PLASMA CONCENTRATION OF FSH IN MALE RATS

在电镜下观察了雄性大鼠腹腔注射氯化镉(2mg/kg)后7、15、30、50天垂体促性腺细胞的变化,并对电镜形态计量学所得的细胞结构参数和血浆FSH放射免疫测定值进行了统计分析。结果表明,促性腺细胞未见损伤,而呈去势表现。其中以15天组最为明显,以后逐渐恢复。主要是粗面内质网扩大,数目增多,内含低电子密度物质。各实验组大、小分泌颗粒数密度、体密度均有明显减少。大、小分泌颗粒直径变化不明显,线粒体的面数密度和体密度变化也无显著性。实验组中,高尔基复合体增生、肥大明显,促性腺细胞也呈肥大、增生表现。血浆FSH测定表明,注射镉7天后FSH分泌增加,15天达到高峰,50天仍未完全恢复。结果指出,垂体促性腺细胞对镉不敏感,出现肥大、增生、合成、分泌旺盛是由于镉对睾丸的化学去势反馈引起。

This paper presents the ultrastructrual changes of male rats gonadotrophs at 7, 15, 30, 50 days after cadmium chloride (Cd) injection (2mg/kg). The differences of morphometric parameters of gonadotrophs and plasma value of FSH were revealed by statistics.The results showed that no injured alterations were found in gonadotrophs but appeared castration like features. These changes feature were most prominent after 15 days and then recovered to normal gradually. The main alterations of gonadotrophs were dilatation, and proliferation and decrease in the specific surface of RER as well as there were low density materials in the dilated cisterna. The numerical and volume density of large and small secretion granules were decreased greatly in all of the treatment groups. The variance of diameter of the large and small secretion granules were not conspicuous and the differences of volume and numerical density of the mitochondria among the control and treatment groups were also no significance. The hypertrophy of Golgi complex of the gonadotrophs were very obvious and the number of the gonadotrophs were increased in the Cd treated rats. The plasma value of FSH measurement indicated that the FSH level increased after 7days and the highest value appeared after 15 days. However, it did not return to normal after 50 days. These results suggested that gonadotrophs were not susceptible to Cd. The hypertraphy, hyperplasia and increase in synthesis and secretion of the gonadotrophs resulted from feedback by the effect of Cd induced injury on testes.