摘要
本研究使用WGA-HRP垂体后叶注射、逆行追踪与免疫组织化学相结合的方法,直接证实了大鼠垂体后叶生长抑素阳性纤维的起源。它们主要分布于前大细胞室旁核到后大细胞室旁核平面的室周区;单个双标记细胞可出现在前连合平面或穹窿后核平面的室周区,个别双标记神经元位于后大细胞室旁核内侧部和内侧小细胞室旁核。室周双标记细胞主要位于室管膜下方,一些挤到室管膜层内,但不直接与管腔接触,其表面有薄层组织与管腔相隔。
WGA-HRP retrograde tracing technique combined with immunohistochemical method was used to study the source of somatostatin postitive fibers in the posterior pituitary. Retrogradely labeled somatostatin immunoreactive cells distributed mainly in the periventricular area from the level of the anterior to posterior magnocellular paraventricular nucleus; single double labeled cells were also found in the periventricular areas at the level of the anterior commissure and posterior fornical nucleus. A few double labeled cells were seen in medial parvocellular paraventricular nucleus and the medial part of posterior magnocelluar paraventricular nucleus. The double labeled cells in the periventricular area lie mainly beneath the ependymal layer. Some were seen to intercalated in-between the ependymal cells, bringing themselves very close to the cerebrospinal fluid, but no direct fluid contacing elements were verified.
出处
《解剖学报》
CAS
CSCD
北大核心
1991年第4期362-365,T003,共5页
Acta Anatomica Sinica
关键词
生长抑素
下丘脑
WGA-HRP
Somatostatin
Hypothalamus
Posterior pituitary
Immunohistoche-mistry
WGA-HRP
Rat