一级感觉神经元周围突分支支配多个内脏器官的研究——荧光素逆行双标及三标法

AN INVESTIGATION OF SENSORY NEURON WITH DICHOTOMIZING PERIPHERAL PROCESSES SIMULTANEOUSLY INNERVATING OF TWO OR THREE VISCERA——A FLUORESCEIN TRACING STUDY

将大鼠颈、胸、腹、盆腔多个脏器配对:食管-甲状腺、食管-气管、心-胃、肺-胃、左肾-脾、膀胱-直肠、膀胱-子宫、子宫-直肠。在配对脏器中先后注入碘化丙啶(PI)或快蓝(FB)及双苯甲亚胺(Bb)或核黄(NY),于迷走神经上、下节及相应背根神经节内出现PI与Bb(或FB与NY)的双标细胞。另设膀胱-子宫-直肠组,分别注入PI、FB及NY,在L_(1～2)背根节出现极少量PI、FB与NY三标细胞。结果提示,大鼠体内普遍存在内脏-内脏感觉在同一感觉细胞的汇聚。

The primary sensory neuron simultaneously innervating two or three viscera was studied in this paper, In one group the viscera in neck, thorax, abdomen and pelvis of the rat were paired off, i.e. esophagus-thyroid gland; esophagus-trachea; heartstomach; lung-stomach; left kidney-spleen; urinary bladder-uterus; urinary bladderrectum; uterus-rectum. Propedium iodide (PI) or fast blue (FB) and bisbenzimide (Bb) or nuclear yellow (NY)were injected separately into the first and second organ of paired viscera.PI-Bb (or FB-NY) double labelled cells were observed in corresponding ganglia (dorsal root ganglia and upper and lower ganglia of vagus nerve). In another group, PI, FB and NY were injected into urinary bladder, rectum and uterus separately. We observed a very few of PI-FB-NY triple labelled cells in L_(1-2) dorsal root ganglia.The above findings indicated that there are sensory convergences of visceroviscera in the same sensory ganglion cell in rat.