摘要
本文介绍了用96孔细胞培养板加入楔形小玻片培养肺泡巨噬细脆的新方法.采用这种培养方法,从每只大鼠的肺灌洗液中可获得25～75张肺泡巨噬细胞爬片样本,能广泛用于组化染色及光、电镜观察,可有效地对肺泡巨噬细胞的吞噬、粘附、融合、细胞毒等作用进行研究。极大地节省人力和物力.
A new method of alveolar macrophage culture by putting a small slide in flat bottom well is described.By this method, 25-75 adhered samples of monolayer culturine alveolar macrophage can be obtained from one lavaged rat.It can be widely used in the study of various morphology and cytochemistry and sparely in the materials and resources, then it is worthy extending.
出处
《解剖学杂志》
CAS
CSCD
北大核心
1991年第4期381-383,F003,共4页
Chinese Journal of Anatomy
关键词
肺泡
巨噬细胞
细胞培养
alveolar macrophage, cell culture.
