二次微分简易示波伏安法用于葛根素测定

Determination of Puerarin by Second Order Differential Simple Oscillographic Voltammetry

根据在 0 .1 mol/ L Na OH溶液中 ,葛根素在二次微分简易示波伏安图阴极支-0 .95V ( vs.SCE)产生一灵敏峰的示波特性 ,建立了二次微分简易示波伏安法测定药品中葛根素含量的新方法。线性范围为 2 .5× 1 0 - 6～ 3 .5× 1 0 - 5mol/ L,相关系数为 0 .9985,检出限为 1× 1 0 - 6mol/ L,对 9.0× 1 0 - 6mol/ L葛根素进行 5次测定的RSD为 1 .5%。利用该法测定中药葛根及心可舒片剂中葛根素的回收率分别为 1 0 0 .2 %和 96.9%。

A sensitive incision of puerarin on the cathodic branch of dE/dt-E curve was observed in 0.1 mol/L NaOH. The incision potential was -0.95 V (vs. SCE). The peak height on the second order differential simple oscillogram was proportional to the concentration of puerarin over 2.5×10 -6~3.5×10 -5 mol/L. The detection limit and the relative coefficient of the calibration curve were 1×10 -6 mol/L and 0.9985,respectively. The recoveries of puerarin in (Wild) Ohwi and Xin Ke Shu tablet were 100.2% and 96.9%, respectively. Compared with other methods, simple oscillographic voltammetry with second order differential was a convenient method due to its cheap equipment and rapid measurement and no need for O 2-eliminated.