毛细管电泳用β-环糊精作改性剂分离血清中吗啡和可待因

Separation of Morphine and Codeine in Serum by Capillary Electrophoresis with β-CD as an Additive

建立了血清中吗啡和可待因的毛细管电泳分离分析方法。讨论了电泳分离模式、缓冲液 p H、β-环糊精 (β-CD)浓度对分离的影响。吗啡和可待因检测限均为 0 .1μg/ m L,不同浓度的萃取回收率分别大于 92 %和 93 % ,迁移时间的相对标准偏差分别小于 0 .44 %和 0 .3 9% ,峰面积的相对标准偏差分别小于 3 .2 %和 4.1 %。

A method for the separation of morphine and codeine in serum by capillary electrophoresis(CE) with β-CD as an additive was established. Expriments were performed with 15 kV power, 50 μm×35 cm fused-silica capillary column and the absorption detection of 214 nm. Sample were injected into the capillary by 4 kV for 5 s and the running buffer was made up of 10 mg/mL β-CD and 40 mmol/L Tris-H 3PO 4(pH 4.00). The influence of mode of CE, pH of buffer and β-CD concentration in the buffer has been studied. The recoveris of morphine and codeine were more than 92% and 93% and the LOD were 0.1 μg/mL all. The RSD% of migration time was less than 0.44 and 0.39, but the RSD% of peak area was less than 3.2 and 4.1, respectively.