肿瘤坏死因子对大鼠纤维连接蛋白的作用

EFFECT OF RECOMBINANT TUMOR NECROSIS FACTOR ON FIBRONECTIN

静脉注射肿瘤坏死因子(TNF,30μg/100g体重)后,大鼠血浆纤维连接蛋白(Fn)含量逐渐降低(与实验开始时比),1/2h时最明显(P<0.05)。对照组无明显变化。血浆Fn与TNF一起体外孵育未见Fn降低,因此TNF的上述作用似非直接。与此同时,组织Fn含量及免疫荧光强度均增加,尤以肾内变化为显。由于血浆Fn降低,而组织Fn升高,因此TNF通过增强微血管通透性导致血浆Fn向组织转移的可能性有待进一步证实。

The content of plasma fibroneotin (Fn) gradually decreased after intravenous injection of tumor necrosis factor (TNF 30μg/10 body weight). At 2 hours after administration, the decrease was most significant (P<0.05) in TNF group (n = 6), but there was no marked changes in the control group (n = 6). When plasma Fn was incubated with TNF in vitro, no decrease of plasma Fn content was found. It seemed that the above mentioned effect is not direct, Meanwhile, the content of tissue Fn and the Intensity of Fn immunofluoresoet noe in tissues both increased, particularly in the kidney. The possibility that TNF increases miorovascular permeability and then plasma Fn moves into the tissues is to be further proven.