小鼠输卵管卵母细胞的形态学研究

Morphological Studies on the Mouse Oviductal Oocyte

应用光、电镜技术研究发现:新排卵母细胞的纺锤体位于动物极质膜下,长轴与质膜平行,卵周隙狭窄,除纺锤体区外,微绒毛呈倒伏状遍布于卵细胞表面,皮质颗粒排列于质膜下,线粒体多分布于卵母细胞近中区及染色体旁;排卵后2～4h,纺锤体在质膜下旋转90°,卵周隙增宽,微绒毛逐渐竖起,少数皮质颗粒提前外排,线粒体趋于分散,排卵后8h,纺锤体内移,微绒毛趋于融合,皮质颗粒减少,线粒体退化。

The main findings with the techniques of light and electron -mi-croscopes(SEM and TEM) were shown as follows; the spindle of freshly ovu-lated oocytes is located under the animal pole cytoplasmic membrance, and its axis is oriented parallel to the egg plasma membrance. Microvilli lay over the entire egg surface with the exception of the region overlaying the spindle, The perivitelline space is narrow. Simple layer cortical granules distribute irregularly throughout the peripheral zone of the oocyte with the exception of the region overlaying the spindle. Vesicular mitochondria are mainly located at the central area of the oocyte and at the side of chromosomes. The metaphase II spindle rotates 90?under the egg plasma membrane 2-4 hours after ovulation. Perivitelline space becomes wide. Microvilli set upright and become long and thin. Few cortical granules release their contents to the perivitelline space in advance. Mitochondria tend to disperse. The spindle migrates inward 8 hours after ovulation. Microvilli tend to mix and become wide. The number of cortical grannules reduces, and and mitochondria degenerate.