几种体外培养条件下间日疟原虫红内期裂体增殖

ERYTHROCYTIC SCHIZOGONY OF PLASMODIUM VIVAX UNDER VARIOUS CONDITIONS OF IN VITRO CULTIVATION

在培养液成分基本不变的情况下,观察几种体外培养条件的间日疟原虫红内裂体增殖.实验组为:烛缸静止培养,重复点烛烛缸静止培养,低氧静止培养,两种试管深层悬浮培养。引种用了两个虫株,起始培养采用冷冻虫血复苏和现症疟疾病人新鲜虫血直接引种。除棉塞试管深层悬浮组不能支持裂体增殖外,其他组均至少不同程度地完成两个裂殖周期;在低氧下,原虫发育更正常。观察发现,间日疟原虫分离株在体外培养中是重要的。

This study was performed to observe the erythrocytic schizogony of P. vivax under several culture conditions in vitro. Five experimental groups included: 1) static cultivation in candle jar; 2) static cultivation in candle jar with candle relightened; 3) static cultivation under low oxygen tension (5-10%); 4) deep suspension cultivation in test tubes with cotton plunger; 5) deep suspension cultivation in closed test tubes with screw cap. Two different isolates of P. vivax collected from Jingshan County and Zaoyang County were used. Cultivation was initiated with two methods, i.e. direct inoculation from fresh patient blood with malaria parasites and retrieval cultivation from freezing malaria parasite blood. The suspension cultivation in test tube with cotton plunger could not support the schizogony of P. vivax, while other groups could at least complete two schizogony cycles. The best result was obtained with static cultivation under low oxygen tension, the growth of parasites appeared to be more normal. The results showed that cultivation of P. vivax under a low oxygen concentration of 5-10% is preferred and the selection of isolates of P. vivax might be important in in vitro cultivation.