反相高效液相色谱法测定体液中哌拉西林浓度及其药代动力学研究

RP-HPLC method for the determination of piperacillin concentrations in human pl asma and urine and its application to clinical pharmacokinetic study

目的 :建立测定体液中哌拉西林浓度的 HPLC法并应用于人体药代动力学研究。方法 :8名志愿者单剂量静脉滴注 4g哌拉西林钠 ,采用 RP-HPL C法测定血浆和尿中药物浓度。 结果 :滴注完成即刻 cm ax为 (2 0 3 .2 4± 44 .13 )μg/ml,t1 / 2β为 (0 .76± 0 .0 9) h,分布容积 (Vc)为 (2 0 .64± 4.67) L,清除率 (Cls)为 (2 4.86± 4.13 ) L/h,AUC为 (2 15 .5 2±3 8.75 ) μg·h·ml-1。结论 :哌拉西林的体内过程符合二室开放模型 ,主要以原形经肾脏排出体外 ,8h累积尿排百分率为(61.0 0± 5 .5 5 ) %。

Objective:To establish a RP-HPLC method for th e determination of piperacillin concentrations in body fluids, and to investigat e the pharmacokinetics of piperacillin in healthy volunteers.Methods: Eight healthy volunteers were given a single dose of piperacillin sodiu m 4 g by infusion.Piperacillin concentrations in plasma and urine were measured by RP-HPLC method. Results:The peak plasma level ( c max ), plasma elimination half-life ( t 1/2β ),volume of distribution ( V c), clearance (Cls) and area under plasma concentration-time curve (AUC) were (203.24±44.13)μg/ml, (0.76±0.09)h, (20.64±4.67) L , (24.86±4.13) L/ h and (215.52±38.75)μg· h·ml -1 , respectively.Conclusion:The disposition of piperacillin can be discribed with a two compartment o pen pharmacokinetic model. It is mainly excreted through kidney in unchanged for m. The cumulative urinary excretion rate of piperacillin is (61.00±5.55)% in 8 h after administration.