化痰平喘方灌胃与直肠给药药效作用的实验研究

Experimental study on pharmacodynamics of Sputum-resolving and Asthma- relieving Formula by intragastric or rectal administration

目的 :比较化痰平喘方不同给药途径的药效。 方法 :采用对小鼠或大鼠灌胃及直肠给药的途径 ,设人体剂量的 5倍、10倍、15倍 3个剂量组 ,分别以急支糖浆、地塞米松、祛痰灵作为阳性对照组 ,用小鼠耳廓肿胀法 (急性炎症 )、大鼠塑料环肉芽肿法 (慢性炎症 )、小鼠气管酚红法等实验方法 ,作药效比较。结果 :灌胃、直肠给予生药 6.5 g· kg-1·d-1组均能抑制小鼠耳廓肿胀 (P<0 .0 5、P<0 .0 1) ;灌胃、直肠给予生药 13 g· kg-1·d-1组均能抑制大鼠肉芽增生 (P<0 .0 5、P<0 .0 1) ;灌胃、直肠给予生药 6.5 g· kg-1 · d-1 组均能促进小鼠排痰 ,而且直肠给药组作用优于灌胃组 (P<0 .0 5 )。结论 :灌胃、直肠给予化痰平喘方组均有抗急、慢性炎症作用 ,两者无显著差异 ;

Objective: To compare the pharmacologic effects of Sputu m-resolving and Asthma-relieving Formula through different routes of administr ation. Methods: The functions of Sputum-resolving and Asthma- relieving Formula were observed by intragastric or rectal administration to mi ce or rats.The test methods were auricular tumefaction in mice, plastic ring gr anuloma in rats and tracheal phenol red test in mice. Results: B oth intragastric and rectal administration of 6.5 g·kg -1 ·d -1 cou ld inhibit auricular tumefaction in mice and granuloma hyperplasia (13 g·kg -1 ·d -1 ), and could improve phenol red excretion rate (6.5 g·kg -1 ·d -1 ). The pharmacological effects of Sputum-resolving and Asthm a-relieving Formula by rectal administration were better than by intragastric a dministration in phenol red test ( P <0.05). Conclusion: Both intragastric and rectal administration of Sputum-resolving and Asthma-relievi ng Formula can inhibit acute or chronic inflammation in mice or rats.