大鼠肺癌前病变及其γ-谷氨酰转肽酶表达的研究

Precancerous and Cancerous Lesions and Their Gamma- glutamyl transpeptidase Expression in 3-Methylcholanthrene- Induced Lung Carcinogenesis in Rats

用3-甲基胆蒽(3-MC)诱发大鼠肺癌前病变,并观察癌变过程中γ-谷氨酰转肽酸(GGT)的组织化学反应。结果支气管内1次灌注3-MC(3mg/100g体重)30d后,大鼠小支气管上皮不典型增生、腺样增生与腺癌及鳞癌的发生率分别为88.2％、70.6％及17.7％,60d的发生率分别为83.3％、23.5％及38.9％,且上述病变均呈GGT染色阳性反应,提示GGT可作为肺癌前病变的标志酶之一。

Lung precancerous and cancerous lesions were induced in Wistar rats by one intrabroncheal injection of 3- methylcholanthrene (3-MC). The incidences of atypical hyperplasia of bronchiolar epithelium, adenoid hyperplasia or adenomas, and squamous cell carcinoma were 88.2%(l5/17), 70.6%(l2/17)and 17.7% (3/17) respectively by the 30th day af.er 3-MC treatment,and 83.3% (15/18), 23.5% (4/17) and 38.9% (7/18)by the 60th day.Positive expression of gamma-glutamyltranspeptidase (GGT) was identified histochemically in all of the above precancerous lesions and carcinomas. GGT might be used as a marker of precancerous and cancerous lesions of the lung.