雌性激素体外诱发小鼠胚胎细胞肿瘤性转化的进一步研究

Further Study of Neoplastic Transformation Effect of Estrogenic Hormones on Mouse Embryo Cell in vitro

本文进一步观察戊酸雌二醇(E)和已酸孕酮(P)两种成分单独应用是否对小鼠胚胎细胞有致癌作用,同时与促进剂TPA联合应用是否会增强其致癌性。 EP、E、P、EP+TPA、E+TPA、P+TPA组细胞均有转化灶形成。抗乌哇巴因抑制生长试验阴性,说明细胞无Na~+/K~+ATP酶位点突变。转化细胞在软琼脂培养基有细胞集落形成。未见TPA明显增强E和/或P的致癌性。上述结果表明雌性激素诱发细胞转化过程,不是通过细胞突变,而可能是通过阻断核分裂中期,形成多倍体,导致细胞转化。

In this paper, further studies on whether estradiol valerate (E) or hydroxypro-gesterone caproate (P) used alone has carcinogenic effect on mouse embryo cells or enhancement of TPA to estrogenic hormones in carcinogenicity were performed. Some morphological transformation fori have been present in some bottles of EP, E, P, EP plus TPA. E plus TPA and P plus TPA. No ouabain-resistant cells were present in any group mentioned above and tested on the 7th day after transformation. It means that there is no mutation at the locus of Na~+/K~+ ATPase. The transformed cells were able to form colonies in soft-agar medium. The results suggest that not only EP but also E or P alone possesses the carcinogenetic activity. In the process of neoplastic transformation induced by estrogenic hormones, it is possible that polyploid growth which leads to the transformation of the cells formed first. No evidence of passing through cellular mutation was noted.