革螨、恙螨细胞培养及其特征的初步研究

Cell Culture of Chigger Mites,Gamasid Mites and Primary Study on their Characteristics

目的 建立螨类细胞培养方法 ,为从细胞分子水平研究革螨、恙螨作为HFRS媒介提供条件。方法 采用革螨、恙螨的若虫、幼虫进行螨类细胞培养 ,建立螨细胞系。革螨、恙螨若虫、幼虫经消毒、胰酶消化处理后 ,接种于含15 %FBS改良TC199培养基中培养。结果 两种螨细胞 2周后细胞生长良好 ,并形成单层 ,现已传了 4代。细胞形态以梭形为主 ,染色体 2n =6 ,细胞对数期群体倍增时间 2 0 .5 0h。最能适应在TC199培养基上生长。动态观察对氨基酸要求 ,发现谷氨酸、酪氨酸、鸟氨酸 ,胱氨酸和精氨酸需量最大。结论 本结果为研究HFRSV在螨体内增殖。

Objective To establish cell culture method of the gamasid mites in order to study on the gamasid mites and chigger mites as the vectors of HFRS at the cell molecular level.Methods The mite cell culture derived from the larva and nymph of the gamasid mites and chigger miteswas established.After sterilization and trypsinization,the treated larva and nymph cells were inoculated into the modified TC199 medium containing 15%FBS.Results The cells grew vigorously and formed a single layer after two weeks cultivation,and were stably passed for four generation.The majority of the cells exhibited a morphology of spindle shape,with chromosome 2n=6 and population doubling time 20.50 h.It was found that TC199 medium was quite suitable for the cell growth.Glutamic acid,tyrosine,ornithine,cystine and arginine were found essential while their needs for amino acids were observed dynamically.Conclusion The results provided a theoretical foundation for study on the proliferation and location of HFRSV in mites.