酒精酵母的紫外诱变呼吸缺陷型突变株的筛选

Screening of respiration deficiency mutants of yeasts (Saccharomyces cerevisiae) mutated by ultraviolet lights

通过对酒精酵母进行紫外诱变及 2 ,3,5 -氯化三苯基四氮唑 (TTC)鉴别性培养 ,获得了 2 6株呈白色菌落表型的呼吸缺陷型突变株。诱变条件为 :紫外 (灯管功率 15 W)波长 2 5 3. 7nm ,照射距离 2 1cm ,照射时间为 1m in、 3min分别获得突变菌株 15和

We report that about 26 mutant strains of yeasts (Saccharomyces cerevisiae) which are all respiration deficiency mutants,whose trait is white colony phenotype on the condition of the selective culture of the TTC medium.The ultraviolet lights (UV) mutation condition lies that light strength is 15W,vertical distance is 21cm,wave length is 253.7nm and shining time respectively is 1 minute and 3 minutes,on which the number of the interest respiration deficiency mutant strains acquired respectively is 15 and 11.The acquisition of the target respiration deficiency mutant strains will make it possible to do further research of cytoplasmism genetics and give us a direction to select a strain of yeast ( Saccharomyces cerevisiae ) with the ability of high alcoholic productivity and duration of high temperature.