不孕症患者子宫内膜白血病抑制因子mRNA表达的研究

Study on leukemia inhibitory factor mRNA expression in endometrium of infertile women

目的 研究白血病抑制因子 (LIF)mRNA在黄体功能不全、不明原因不孕及输卵管原因不孕患者子宫内膜的表达 ,了解其与各种不孕症的关系。方法 采用逆转录聚合酶链反应 (RT -PCR)测定不孕患者和正常妇女黄体中期子宫内膜LIFmRNA的表达水平。结果 LIFmRNA在正常妇女 (对照组 )黄体中期子宫内膜的表达水平为 1.12± 0 .45 ,黄体功能不全患者子宫内膜的表达量为 0 .6 4± 0 .41,两者差异有显著性 (P <0 .0 5 ) ;不明原因不孕组表达量为 0 .5 2± 0 .41,与对照组之间差异有显著性 (P <0 .0 1)。输卵管原因不孕组LIF水平为 0 .99± 0 .35 ,与对照组相比差异无显著性 (P >0 .0 5 )。结论 LIFmRNA在黄体功能不全和不明原因不孕患者子宫内膜中表达量降低 ,因此 ,LIF基因表达缺失可能与不孕症的发病有关。

Objective To study the expression of leukemia inhibitory factor (LIF) in the endometrium of infertile women (including luteal phase dysfunction, infertility of idiopathic and oviduct origin), and to explore the relevance of LIF and infertility. Methods The infertility patients came from Gynecology & Obstetrics Hospital of Fudan University. and the LIF mRNA levels in the endometrium of midluteal phase were detected by RT-PCR. Results Semi-quantitative analysis of RT-PCR showed that the LIF mRNA level in the endometrium of normal women ( control group) was 1.12±0.35. while that expressed in the endometrium of luteal phase dysfunction was significantly lower than that of the normal endometrium (P<0.05), The LIF mRNA level of infertility of oviduct origin was close to normal level (P>0.05), whereas that of idiopathic origin decreased (P<0.01).Conclusion These results indicate that defective of LIF gene might be related to infertility of luteal phase dysfunction and infertility.