慢性肝炎患者肝组织内HBeAg的检测及其意义

Detection and Significance of HBeAg in Liver Tissues of Patients with Chronic Liver Diseases

本文采用间接免疫酶标法检测了33例慢性肝病患者肝组织内HBeAg,其中26例慢性肝炎患者肝组织标本中,7例(26.9％)检出HBeAg;7例肝硬化标本中,2例(28.5％)检出HBeAg。HBeAg仅存在于HBsAg和(或)HBcAg阳性的肝组织的病侧中,主要定位于肝细胞浆内与HBcAg关系密切。由此提示HBeAg与HBcAg在肝细胞内同时存在,因为e抗原与HBcAg为同一开读框架,e抗原的本质是HBcAg的降解成分所致。

Indirect immunoenzymatic technique has been employed to detect HBeAg in liver tissues of 33 patients with chronic liver diseases. Our results show that HBeAg has been detected in 7 cases (26.9%) from 26 patients with chronic hepatitis and 2 cases (28.5%) from 7 patients with liver cirrhosis. We also found that HBeAg was expressed only in HBsAg and/or HBeAg positive liver tissues and mainly in the cytoplasma. Its expression was closely associated with HBeAg, suggesting that HBeAg and HBeAg may be expressed simultaneously in cytoplasma. This is consistent with the hypothesis that HBeAg and HBeAg are expressed from one opening reading frame (ORF) and HBeAg is a degenerative part of HBeAg.