应用抗精子抗体检测药盒检测男性不育症血清和精浆抗精子抗体

Determination of Antisperm Antibodies in the Serum and Seminal Plasma of Infertile Men Using the Enzyme-Linked Immunosorbent Assay(ELISA) Kit

本文应用国产酶联免疫吸附测定抗精子抗体(AsAb)检测药盒“Sperm AB”,检测了15例生育男性和82例不育男性的血清和精浆AsAb。结果显示:该药盒特异性好、灵敏度高、操作简易快速。生育和不育者AsAb阳性率分别为6.67％和32.92％(P<0.05),其中不育男性的血清和精浆两者的阴性阳性行合率为75.0％。AsAb阳性与阴性不育者精液量。精子密度和活率均无明显差异。提示在男性不育症的诊断中,采用该药盒测定AsAb较为实用,且对血清和精浆AsAb的同时测定是必要的。

Antisperm antibodies (AsAb) were determined with ELISA kit 'Sperm AB' in the serum and seminal plasma of 15 fertile and 82 infertile men. The results show that this kit is more specific, sensitive and convenient. The positive rate of AsAb in fertile and infertile men was 6.67% and 32.92%, respectively (P<0.05), the negative and positive concidence rate between serum and seminal plasma in infertile men was 75.0%. The AsAb did not affect significantly the density and viability of the spermatozoa in infertile men. From the above it may be suggested that this kit is more helpful in the diagnosis of immunological infertility, and that it is necessary to dele mine AsAb in the serum and seminal plasma simultaneously.