帕金森病患者的外周血淋巴细胞DAT功能检测

Analysis of the Function of DAT in Human Lymphocytes with Parkinson Disease

目的 比较帕金森病 (PD)患者与正常对照组外周血淋巴细胞摄取多巴胺 (DA)量的差异。方法 用密度梯度离心法提取外周血淋巴细胞 ,以 Western blot法检测淋巴细胞上多巴胺转运蛋白 (DAT蛋白 ) ,以液体闪烁记数法检测淋巴细胞摄取 3H-DA的量。结果 Western blot显示在相对分子质量为 660 0 0部位出现一与 DAT蛋白大小相吻合的条带 ;淋巴细胞有摄取 3H-DA的功能 ,且为时间、浓度及 Na+依赖 ;PD患者淋巴细胞摄取 3H-DA的量明显低于正常对照组 (P <0 .0 5 )。结论 检测 PD患者外周血淋巴细胞摄取3H-DA量与正常对照组之间的差异有可能成为

Objective The purpose was to compare the quantity of uptaking of DA by DAT in human lumphocytes between patients with PD and normal controls. Methods Human lymphocytes were isolated on a Ficoll Hypaque gradient and the DAT protein were detected by the way of Westernblot. The quantity of uptaking of DA was detected by the way of liquid scintillation counting. Results There exist the protein band with Mr 66 000 similar to DAT in Lymphocytes. The uptaking of 3 H DA by human lymphocytes was time, temperature and Na + dependent.The quantity of uptaking of 3H DA by human lymphocytes were significantly lower in patients with PD than that in normal controls(P<0.05). Conclusions The difference of uptaking of 3H DA by human lymphocytes between patients with PD and normal controls might have some diagnostic value for PD.