22例围排卵期卵泡液生化成份分析及意义

Analysis of biochemical components in follicular fluid from 22 cases during periovulatory period

目的 通过对人围排卵期卵泡内卵泡液生化物质的分析并与血清相应物质水平进行比较,初步探讨卵泡液的形成与体液循环的关系,了解卵子生长的必需要素,为IVF-ET工作中IVM培养液的配制提供参考。方法 采用全自动生化分析仪（日立7150型）对22例在本中心接受ART女性的卵泡液及血清中的8种酶、7项脂类及蛋白质、葡萄糖等25项指标进行测定。结果 卵泡液中有13种生化物质与血清含量无差异（P>0.05）,葡萄糖、总胆固醇及甘油三酯等明显低于血清含量,差异有非常显著意义（P<0.001）;LDL-C、APO-B、LP（a）等脂类在卵泡液中含量甚微,有的几乎为零:AST、LDH、CK-MB水平升高有显著意义（P<0.001）。结论 卵泡液内的生化成份主要来源于血清,证明卵泡液的形成与体液微循环密切相关,可为在体外培养配制或改进更适合卵子（特别是未成熟卵子）生长的培养液提供一定的参考。

Objective To explore the relationship between the formation of follicular fluid and humoral circulation,and to study the essential factors for the growth of ovum. Methods Follicular fluid and serum were individually collected from 22 women undergoing ART treatment.Twenty five indices inclading the concentration of 8 enzymes,7 lipids and proteins and glucose were assayed by automatic analyzer (HITACH 7150 ). Results There was no statistical significance between the concentrations of 13 kinds of biochemical substances in follicular fluid and those in serum (P>0.05). The contents of glucose and total cholesterol in follicular fluid were significantly lower than those in serum (P<0.001).The LDL-C, APO-B, LP(a) levels in follicular fluid were almost zero. The AST、LDH、CK-MB levels in follicular fluid elevated significantly (P<0.001). Conclusions The biochemical components in follicular fluid originate largely from serum. The results suggest that the formation of follicular fluid correlates well with humoral circulation.