HBeAg、HBeAb双阳性患者血清中病毒前C区基因序列分析

THE SEQUENCE ANALYSIS OF HBV GENE ISOLATED FROM BOTH HBEAG AND HBEAB POSITIVE PATIENTS

目的 了解乙型肝炎病毒e抗原和e抗体双阳性患者血清中病毒前C区基因的变异情况。方法 采用时间分辨荧光免疫分析方法检测乙肝病毒免疫标志物 ,对HBeAg、HBeAb双阳性患者采用聚合酶链反应 (PCR)扩增其血清中HBVDNA前C区基因 ,然后对阳性样品的PCR产物直接标记测序 ,并和Genbank中登录的代表株进行比较分析。结果 1 5例HBeAg、HBeAb双阳性患者中有1 1例HBVDNA阳性 ,序列分析显示所有阳性血清中病毒前C区基因均发生了变异 ,其中有 4例存在A1 896变异。结论 在HBeAg和HBeAb血清学转换过程中 ,均伴有HBV前C区基因变异 ,A1 896变异的产生主要在HBeAb产生过程中或产生以后。

Objective To investigate HBV precore variants in patients with HBeAg and HBeAb positive. Methods HBV markers were detected by DELFIA. HBV precore gene were amplified by polymerase chain reaction and sequenced directly in ABI 310 machine. The precore gene sequences were compared with the representative sequences in Genbank. Results 11 of 15 patients were positive for HBV DNA. The sequence analysis showed that variants existed in all sequences, and A1896 mutations existed in 4 out of 11 patients. Conclusion There are precore gene variants in patients who have HBeAg/HBeAb seroconversion, and A1896 mutations may be detected after HBeAb in patients with hepatitis B virus infection.