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Determination of Acrylamide Metabolite,Mercapturic Acld by High Performance Liquid Chromatography 被引量:4

Determination of Acrylamide Metabolite, Mercapturic Acid by High Performance Liquid Chromatography
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摘要 A HPLC Assay was developed to identify and measure the metabolite of acrylamide, mercapturic acid, N-Acetyl-s-(propionamide)-cysteine (APC) in urine. O-phthalaldehyde (OPA) was utilized as a precolumn derivatizing agent in the assay. This derivative was isolated with a good selectivity by high performance liquid chromatography (HPLC) employing reversed phase ODS columns. The quantitation of the mercapturic acid derivative was reproducible and with a detection limit of 1 pmol. The average coefficient of variation for the runs carried out on the same day was approximately 4.6% at the range of 80-160 ianol-L'1 of APC, and the mean analytical recovery from urine samples was 94%.The metabolite of urine of workers exposed to acrylamide was identified as N-acetyl-s-(propionamide)- cysteine by HPLC chromatography and fluorescence scan and HPLC-Mass spectra. All results were identical with the authentic synthesized compound. A HPLC Assay was developed to identify and measure the metabolite of acrylamide, mercapturic acid, N-Acetyl-s-(propionamide)-cysteine (APC) in urine. O-phthalaldehyde (OPA) was utilized as a precolumn derivatizing agent in the assay. This derivative was isolated with a good selectivity by high performance liquid chromatography (HPLC) employing reversed phase ODS columns. The quantitation of the mercapturic acid derivative was reproducible and with a detection limit of 1 pmol. The average coefficient of variation for the runs carried out on the same day was approximately 4.6% at the range of 80-160 ianol-L'1 of APC, and the mean analytical recovery from urine samples was 94%.The metabolite of urine of workers exposed to acrylamide was identified as N-acetyl-s-(propionamide)- cysteine by HPLC chromatography and fluorescence scan and HPLC-Mass spectra. All results were identical with the authentic synthesized compound.
出处 《Biomedical and Environmental Sciences》 SCIE CAS CSCD 1993年第3期273-280,共8页 生物医学与环境科学(英文版)
基金 Project supported by the National Natural Science Foundation of China.
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