摘要
目的探讨SH3PXD2B蛋白介导的巨噬细胞的趋化性在中耳炎发病机制中的可能作用。方法引入SH3PXD2B突变型小鼠-/-及野生型+/+小鼠,采用听觉脑干诱发电位,耳内镜筛选、剔除。免疫组织荧光法进行F-肌动蛋白及SH3PXD2B抗体双重染色,采用硫代乙醇酸盐培养基(thioglycollate,TG)诱出法获得腹腔巨噬细胞,进行在体及离体巨噬细胞的趋化游走能力检测,并进行中耳腔巨噬细胞诱出率的比较。结果双重染色结果F-动蛋白及SH3PXD2B蛋白均表达于树突状结构,且位置一致;离体及在体巨噬细胞的趋化游走能力检测,突变型-/-巨噬细胞游走能力下降,突变型-/-小鼠中耳腔巨噬细胞的诱出率下降。结论 SH3PXD2B突变导致树突状结构形成障碍,进而影响巨噬细胞的趋化游走功能可能是中耳炎发病机制之一。
OBJECTIVE To explore the possible role of SH3PXD2 B mediated-Chemotaxis of macrophage i n me ch a n ism of ot it is me d ia. M ET HODS Twe nt y SH3 PX D2B mut ant m ice and 20 wild-t y pe lit ter mate mice were used in this study. Immunofluorescent staining methods was used to stain F-actin and SH3PXD2 B. TG method was used to elicite macrophages from abdomen, and capability of Chemotaxic migration between mutant and wildtype macrophage was compared in vivo and in vitro. And then the elicited rate of macrophage to middle ear between mutant and wildtype mice was compared too. RESULTS Co-staining of F-actin and SH3PXD2 B showed that the expression of SH3PXD2 B localized in podosome. The capability of Chemotaxic migration of macrophages in vivo and in vitro showed that the capability of Chemotaxic migration in mutant macrophages was weaker than that in wildtype macrophages, as well as the elicited rate of macrophage in middle ear. CONCLUSION The mutation of SH3PXD2 B influnce the formation of podosome and the capability of chemotaxic migration of macrophage maybe one of the mechanisms of otitis media.
出处
《中国耳鼻咽喉头颈外科》
CSCD
2015年第2期92-96,共5页
Chinese Archives of Otolaryngology-Head and Neck Surgery
基金
广东省医学科研基金资助项目(B2014135)
