肿瘤血管生成相关因子CD105和β-半乳糖凝集素3在喉鳞状细胞癌中的表达

Expressions of tumor angiogenesis related factors CD105 and Galectin-3 in laryngeal squamous cell carcinoma and biological assessment

目的探讨喉鳞状细胞癌(laryngeal squamous cell carcinoma,LSCC)中Endoglin(CD105)、β-半乳糖凝集素3(Galectin-3)蛋白的表达及与LSCC临床病理因素的关系。方法采用免疫组化SP法检测76例LSCC和25例喉正常黏膜中CD105、Galectin-3的表达。结果 1LSCC中以CD105标记的微血管密度(microvessel density,MVD)为10.33±2.29,大于喉正常黏膜(1.20±1.04,t=18.732,P<0.05)。CD105标记的MVD(CD105-MVD)的表达与LSCC的组织学分级、T分级、临床分期、淋巴结转移、是否复发、预后有关(P均<0.05)。2Galectin-3蛋白表达的阳性率在LSCC为86.84%,大于喉正常黏膜36%(χ2=25.44,P<0.05)。G a le c t i n-3蛋白表达与L SCC的T分级、临床分期、淋巴结转移、是否复发、预后有关(P均<0.05)。3CD105和Galectin-3蛋白表达呈正相关。4生存分析显示,CD105、Galectin-3、组织学分级、淋巴结转移、T分级、有无复发是患者预后独立的影响因素(P<0.05)。结论 LSCC中CD105与Galectin-3蛋白的表达呈正相关,二者均促进LSCC的发生、恶性发展及不良预后,联合检测两者的表达有助于LSCC的诊断及预后判断。

OBJECTIVE To investigate the expressions of Endoglin(CD105) and Galectin-3 protein in laryngeal squamous cell carcinoma(LSCC) as well as the relationship between their expressions and the clinicopathological factors of LSCC.METHODS The expressions of CD105 and Galectin-3 protein were detected in 76 samples of LSCC and 25 normal laryngeal tissues(NLT) by immunohistochemical staining(S-P).RESULTS1.The mean of Microvessel density(MVD) value marked by CD105 in LSCC was 10.33±2.29,which was significantly higher than that in NLT(1.20±1.04)(P<0.05).The expression of MVD marked by CD105(CD105-MVD) was correlated with histological grading,T stage,clinical stage,lymph node metastasis,recurrence and prognosis in LSCC(P<0.05).2.The positive expression rate of Galectin-3protein in LSCC was 86.84%,which was significantly higher than that in NLT(36%)(P<0.05).The expression of Galectin-3 was correlated with T stage,clinical stage,lymph node metastasis,recurrence and prognosis in LSCC(P<0.05).3.There was apositive correlation between CD105 and Galectin-3 protein.4.Survival analysis indicated that the expressions of CD105 and Galectin-3,histological grading,lymph node metastasis,T stage and recurrence were independent factors for tumor prognosis in LSCC(P<0.05).CONCLUSION The expressions of CD105 and Galectin-3protein have a positive correlation in LSCC.They may play important roles in the tumorigenesis,malignant progression and poor prognosis of LSCC.Combined detection of them may be great value in diagnosis and predicting prognosis of LSCC.