喉癌高危型人乳头状瘤病毒检测及表皮生长因子受体表达的研究

Study of the detection of high risk HPV and EGFR expression in laryngeal cancer

目的检测喉癌组织中高危型人乳头状瘤病毒(HPV)感染比例及表皮生长因子受体(epidermal growth factor receptor,EGFR)的表达,探讨HPV与喉癌之间及HPV与EGFR的关系。方法收集广州市耳鼻咽喉头颈外科医院2010年3月~2016年3月喉癌组织标本136例,声带息肉标本33例,PCR-反向点杂交法检测两组标本中高危型HPV DNA并分型,比较两组之间HPV感染差异,免疫组化测定EGFR在喉癌组织中的表达,研究HPV与EGFR的相互关系。结果喉癌组中HPV感染率为19.8%(27/136),声带息肉组中HPV感染率为3.0%(1/33),两组之间具有明显差异(χ~2=5.4369,P=0.0197),喉癌中HPV感染与年龄、性别、大量抽烟和/或饮酒史、病理分型及肿瘤原发部位无关,无显著性差异(P均>0.05)。喉癌中感染HPV组与未感染HPV组,EGFR蛋白阳性表达率分别为92.6%和61.5%,两者差异具统计学意义(χ~2=9.5785,P<0.05)。结论喉癌中存在着一定比例的HPV感染,HPV与喉癌有一定相关性。喉癌中HPV感染与EGFR的表达呈正相关,HPV感染引起EGFR过表达可能是喉癌的发病因素。

OBJECTIVE To investigate the proportion of high-risk HPV infection and the expression of EGFR in laryngeal cancer,and explore the relationship between HPV and laryngeal cancer,and the relationship between HPV infection and EGFR expression.METHODS Laryngeal cancer tissue specimens of the 136 cases with laryngeal cancer were collected from 2010 to 2016 in Guangzhou Hospital of Otolaryngology Head and Neck Surgery,and specimens of 45 cases with vocal fold polyps were also collected as control.PCR reverse dot hybridization method was used to detect the highrisk type HPV DNA in specimens of the two groups.The expression of EGFR in laryngeal carcinoma was detected by immunohistochemistry,and the relationship between HPV and EGFR was studied.RESULTS The HPV infection rate was 19.8%in the laryngeal cancer group,and 3.0%in the vocal cords polyp group.There was a significant difference between the two groups(P<0.05).There was no significant difference in HPV infection between laryngeal carcinoma and age,sex,history of heavy smoking and/or drinking,pathological classification and primary location of tumor(P>0.05).The positive expression rates of EGFR protein in HPV infected group and uninfected HPV group were 92.6%and 61.5%,respectively.There was significant difference between the two groups(P<0.05).CONCLUSION There is a certain proportion of HPV infection in laryngeal carcinoma,and there is a certain correlation between HPV and laryngeal carcinoma.There is a positive correlation between HPV infection and the expression of EGFR in laryngeal carcinoma.The overexpression of EGFR caused by HPV infection may be the pathogenic factor of laryngeal carcinoma.