摘要
本试验校正了利用肝素和咖啡因使牛精子体外获能的传统的BO液配方及培养方法;利用去透明带仓鼠卵的穿透试验和顶体的台盼兰——姬姆萨双重染色技术检查了该方法对获能的影响,发现校正的BO液培养法缩短了预培养时间,增强了获能效果。
The traditional BO medium and method using caffeine and heparin for the in vitro capacitation of bovine spermayozoa was modified in this study. Zona-free hamster eggs penetration test (HEPT) and Trypan blue-Giemsa stain technique were employed to examine the effect of mBO medium on capacitation and it was found that the new method shortened preincubation time as well as improved capacitation effect.
出处
《华南农业大学学报》
CAS
CSCD
1991年第A11期18-23,共6页
Journal of South China Agricultural University
基金
张天佑教授主持的国家自然科学基金
关键词
牛
BO液培养法
精子
人工授精
Bovine spermatozoa
Capacitation
Zona-free hamster eggs penetration test(HEPT)
Trypan blue-Giemsa stain
