母鸡在排卵周期和抱窝血浆“黄体生成素类似物”和孕酮的浓度变化

VARIATION OF THE PLASMA "LUTEINIZING HORMONE" AND PROGESTERONE CONCENTRATION DURING THE OVULATORY CYCLE AND BROODY PERIOD IN HENS

本文用放射免疫分析法(RTA)测定了泰和母鸡在排卵周期和抱窝的不同生殖阶段,血浆“黄体生成素类似物”(“LH”)和孕酮(P)的浓度。结果表明:母鸡在排卵周期血浆“LH”和P的平均值分别为3.67±0.60ng/ml(x±SE)和459.50±30.87pg/ml,排卵前6～3h,血浆“LH”和P均出现浓度高峰,其峰值比相应的平均值高出1.1和1.3倍,差异非常显著(P<0.01)。抱窝鸡血浆“LH”和P在24h内的平均值分别为2.27±0.22ng/ml和319.46±12.15pg/ml,显著地低于母鸡在排卵周期的平均值(P<0.05),且在24h内的不同时间,未见有浓度高峰出现。

The concentration of 'luteinizing hormone' ( 'LH') and progesterone (P) in peripheral plasma during the ovulatory cycle and broody period of Tai-Ho hens were determined by radioimmunoassay. As a result of this study it was shown that the mean levels of 'LH' and P were 3. 67±0. 60 ng/ml (X±SE) and 459. 50±30. 87 pg/ml respectively during the ovulatory cycle. The levels of 'LH' and P increased and peaked during 6 - 3h prior to ovulation. They were 7. 90± 1. 82 ng/ml and 1 186. 00± 117. 45 pg/ml, respectively.The peak levels of 'LH' and P were significantly higher than that of the mean levels (P<0. 01) .Plasma levels of 'LH' and P during a 24-hr period in brooding hens were 2. 27±0. 22 ng/ml and 319. 96± 12. 15 pg/ml, respectively. They were obviously lower than those of the laying hen during the ovulatory cycle (P<0. 05) . And also no peak in plasma level of these two hormones appeared such as in the preovulatory laying hen.