预适应对豚鼠受科里奥利加速度刺激后脑组织5-羟色胺含量的影响

Effect of adaptation training on brain tissue 5-HT content following Coriolis acceleration stimulation in guinea pigs

目的 观察科里奥利加速度预适应训练对豚鼠受科里奥利加速度刺激后脑组织 5 -羟色胺 (5 - HT)含量的影响 ,从而探讨预适应对抗科里奥利错觉的生理机制。 方法 2 8只健康花色豚鼠 ,随机分成 4组 (各组 n=7) :空白对照组 (对照 组 )、科里奥利加速度预适应训练组 (训练 组 )、角速度训练组 (训练 组 )、单纯科里奥利加速度刺激组 (对照 组 )。在 30 min科里奥利加速度刺激后 ,对豚鼠受刺激后的行为学表现进行观察 ,然后处死 ,用荧光分光光度法测定各脑区 (大脑皮层、脑干及小脑 )的 5 - HT含量。 结果 同对照 组相比 ,其它 3组中枢内的 5 - HT含量均显著增高 (P<0 .0 5 ) ;同训练 组相比 ,训练 组中枢内的 5 - HT含量显著降低 (P<0 .0 5 ) ,而对照 组中枢 5 - HT含量未有显著变化 (P>0 .0 5 ) ;对照 组及训练 组豚鼠 ,科里奥利加速度刺激后均表现迟缓 ,活动明显减少 ,反应迟钝 ,而训练 组豚鼠 ,在刺激后 ,行为活动力及反应性较对照 组、训练 组明显增加。 结论 科里奥利加速度刺激可引起豚鼠大脑皮层、脑干和小脑 5 -羟色胺含量的增加 ,科里奥利加速度预适应训练可减弱其增加的程度。

Objective To observe the effect of adaptation training on brain tissue 5-HT content following Coriolis acceleration stimulation (CAS) in guinea pigs, so as to explore the mechanism of alleviating Coriolis illusion by adaptation training. Methods Twenty eight guinea pigs were randomly divided into 4 groups: blank control group (control group Ⅰ), CAS group (control group Ⅱ), Coriolis adaptation training group (training group Ⅰ), angular velocity adaptation training group (training group Ⅱ). For the later 3 groups, the behaviors of guinea pigs immediately after CAS(30 min) were observed and then the 5-HT content of cerebrum, brainstem and cerebellum were measured by spectrophotofluorometric assay. Results Compared with those in control group Ⅰ, 5-HT contents of various parts of brain in other 3 groups were significantly higher (P<0.05). Compared with those in control Ⅱ group and training Ⅱ group, brain 5-HT contents in training Ⅰ group were significantly lower (P<0.05). The differences between brain 5-HT contents of control Ⅱ group and training Ⅱ group were nonsignificant. The activity and sensitivity to stimulus after CAS were significantly better than those in control Ⅱ group and training Ⅱ group. Conclusion The mechanism of onset of Coriolis illusion and protective effect of Coriolis acceleration training may be related with the fact that CAS can induce increase of brain 5-HT content, and Coriolis acceleration training can alleviate the elevation of brain 5-HT content following CAS.