山莨菪碱对大鼠急性坏死性胰腺炎肾损伤的保护作用

Protective effects of anisodamine on kidney injury following experimental acute necrotic pancreatitis in rats

目的 探讨大鼠急性坏死性胰腺炎 (ANP)肾损伤的机制及山莨菪碱的治疗作用。方法 采用 35 g·L-1(3.5 %)牛磺胆酸钠溶液逆行注射胰胆管建立鼠的ANP模型。观察炎性介质白细胞介素IL 1β、IL 6、肿瘤坏死因子 (TNFα)、内毒素、淀粉酶、磷酯酶A2 (PLA2 )的变化和山莨菪碱的作用。结果 ANP可以诱导炎性介质的明显升高 ,并与肾损伤的严重程度正相关。而山莨菪碱可抑制ANP的炎性介质过度表达 ,减轻肾损伤的程度。结论 炎性介质参与了ANP对肾的损伤 ,山莨菪碱对ANP肾损伤有治疗作用。

Objective To investigate the mechanism of kidney injury in experimental acute necrotic panceatitis (ANP) and protective effects of anisodamine on kidney injury.Methods The role of inflammatory mediators(IL-1β,IL-6, TNFα, PLA2, endotoxin, and amylase) were studied in ANP model established by retrograde injection of 3.5% sodium taurocholate into pancreatic duct, and efficiecy of anisodamine was also observed.Results ANP could induce the obvious increase of the inflammatory mediators, which was positively correlated with the development of kidney injury. Anisodamine could inhibit the overexpression of inflammatory mediators and alleviate the degree of kidney injury. Conclusion Inflammatory mediators might play an important role in ANP progression and anisodamine may be a efficient treatment to prevent the development and progression of kidney injury.