高效液相色谱法测定十全大补丸中芍药苷的含量

Content Determination of Paeoniflorin of Shiquandabu Pill with Reversed-Phase HPLC

目的 :采用高效液相色谱法测定十全大补丸中芍药苷的含量 ,以控制该制剂的质量。方法 :以C1 8化学键合硅胶柱分离芍药苷 ,以乙腈 0 .1%磷酸 (15∶85 )为流动相 ,UV检测波长 2 3 0nm进行测定。结果 :芍药苷峰与其他组分峰的分离度为 3 .9,理论塔板数以芍药苷峰计算为 3 5 44 0 ;方法的平均加样回收率 99.0 % (n =5 ) ;5次独立测定的相对偏差RSD =0 .90 % ,芍药苷在 0 .2 5～ 2 .5 0 μg范围内 ,浓度与吸收面积值呈良好的线性关系。 结论 :本法测定十全大补丸中芍药苷的含量 ,结果准确 ,重复性好。

Objective:To dominate the quality of shiquandabu pill, the content of paeoniflorin in shiquandabu pill was Determined by Reversed Phase HPLC(high performance liquid chromatography). Method:Paeoniflorin was separated on spherisorb C 18 column by using acetonitrile 0.1% phosphoric acid (15∶85) as mobile phase and wavelength detection at 230 nm by UV. Results:The resolution was 3.9; The number of theoretical plates calculaled for paeoniflorin peak was 35 440; The average recovery of the added index was 99.0 %( n =5). RSD obtained by 5 times of determination was 0.90%. There was a good linear relationship between the concentration of paeoniflorin and absorption area value in the range of 0.25 2.50 μg. Conclusion:The method is highly reproducible and accurate.