局部注射维甲酸对异基因小鼠皮肤移植存活时间的影响

Prolonged survival of mouse skin allograft in recipients treated with Retinoic Acid in location

目的研究维甲酸(RA)对于同种异体皮肤移植存活时间的影响,验证RA应用促进局部Ⅱ型跨膜蛋白(FASL)表达。方法将BALB/C小鼠(H-2D)全厚皮片植于C57BL/6小鼠背部,术后1～5d每日皮下注射10～6mol/L,0.2mlRA,观察皮片存活时间。部分受体术后6d切取所植皮片SABC法免疫组化染色。结果1实验组较对照组供体皮片存活时间明显延长(P<0.05);2实验组在的纤维细胞均有FASL阳性表达,对照组无阳性表达。结论维甲酸(RA)能促进局部FASL表达,延长同种异体皮肤移植存活时间。

Objective To investigate the expression of FASL in location treated with Retinoic Acid and the prolongation F57Associa ted Surface antigen(FASL)/F57Associated surface antigen Lig and(FAS)-mediated skin allograft survival.Method Full-thickness skin of BALB /C mouse was transplanted into C 57BL /6mouse,recipients were treated with 10-6M /0.2ml RA after allogra ft everyday for five days,then have clinical and histolo gical examination.Result RA treatment prolongs the survival o f allograft from normal donor skin(P<0.05)and,RA-injected skin showed positive staining of FASL in dermis ,on the o ther hands,control skin didn' t show any positive cells of FASL in dermis.Conclusion RA selectivly upregulates the expre ssion of FASL ,then prolongs the surv ival of skin allograft.