摘要
目的:建立小儿咳喘灵颗粒中6种主要活性成分的一测多评含量测定方法。方法:以绿原酸为内标物,采用高效液相色谱方法,建立其与新绿原酸、隐绿原酸、异绿原酸B、异绿原酸A、4,5-O-二咖啡酰奎宁酸5种成分的相对校正因子,并用该校正因子进行计算,实现一测多评;同时采用外标法对6种成分进行含量测定,通过比较两种方法的结果,验证该方法的准确性。结果:建立的校正因子重复性较好,采用校正因子计算的含量值与外标法实测值偏差较好,相对误差<5%。结论:采用一测多评法测定小儿咳喘灵颗粒中6种绿原酸类成分可行。
Objective:To establish the quantitative analysis of multi-components by single-marker(QAMS)for content determination of six chlorogenic acids in Xiao’er Kechuanling granules.Methods:Emodin was used as the internal substance,HPLC method was adopted,the relative correction factors of emodin and neocyanogenic acid,cryptochlorogenic acid,isochlorogenic acid B,isochlorogenic acid A,4,5-O-dicaffeyl quinine were established,and the correction factor was used to calculate the results,the QAMS was conducted.Meanwhile,the content of 6 components was determined by external standard method.The accuracy was verified by comparing the results of the two methods.Results:The established correction factor had good repeatability.The content calculated by the correction factor had better deviation from the measured value of the external standard method,with relative error<5%.Conclusion:It is feasible to determine the 6 chlorogenic acids in Xiao’er Kechuanling granules by QAMS.
作者
曾桢
刘莉
李峰
文永盛
周世玉
薛丹
Zeng Zhen;Liu Li;Li Feng;Wen Yongsheng;Zhou Shiyu;Xue Dan(Chengdu Institute of Food and Drug Control,Sichuan Chengdu 610045,China;West China School of Pharmacy Sichuan University,Sichuan Chengdu 610041,China)
出处
《儿科药学杂志》
CAS
2019年第5期46-51,共6页
Journal of Pediatric Pharmacy
基金
全国中药资源普查实施四川省试点项目
编号2014SS029-1
关键词
一测多评法
外标法
高效液相色谱法
校正因子
小儿咳喘灵颗粒
含量测定
quantitative analysis of multi-components by single-marker
external standard method
HPLC
correction factor
Xiao’er Kechuanling granules
content determination