摘要
目的研究Apo E基因多态性及其在阿尔茨海默病(AD)早期诊断中的应用。方法应用PCR-RFLP和聚丙烯酰胺凝胶电泳方法检测Apo E基因多态性。结果对照组与AD组之间,ε_2/ε_2、ε_3/ε_3、ε_4/ε_4、ε_2/ε_3和ε_2/ε_4基因型频率以及ε_2和ε_3基因频率间无显著性差异(P>0.05)。ε_3/ε_4基因型频率ε_4基因频率,AD组明显高于对照组(P<0.05)。结论 Apo E基因在连云港地区有遗传多态性,ε_4基因检测可用于AD的早期辅助诊断。
Objective To study the genetic polymorphism of Apo E gene and its application in early disagnoses of the Alzheimer diease (AD) .Methods The PCR- RFLP was used to examine the Apo E genetic polymorphism.Results There are no significantl difference in ε2/ε2、 ε3/ε3 、 ε4/ε4、 ε2/ε3 and ε2/ε4 genotype frequence, also the ε2 and ε3 gene frequences between comparison group and AD group ( P > 0.05) .The ε3/ε4 genotype frequence and ε4 gene frequence of AD group are significantly higher than the comparison group (P<0.05) .Conclusion There are genetic polymorphism of Apo E gene in Lian yun - gang area, and the examine of the Apo E ε4 gene can be used in early diagnoses of the AD.
出处
《医学研究通讯》
2002年第9期6-9,共4页
Bulletin of Medical Research
