小麦吸收土壤磷转运子在酵母突变体中的功能互补分析

Function Analysis of A Wheat Phosphate Transporter in Yeast Mutant

以小麦磷转运子全长编码cDNA (TaPT2 )为探针与小麦基因组DNA进行Southern杂交 ,结果表明 ,在小麦基因组中存在该基因的不同家族成员。另外 ,将TaPT2基因转入酵母突变体MB192中 ,以野生型菌株YPH0 84为对照 ,分别检测YTaPT2、YPH0 84和MB192酸性磷酸酶分泌情况、生长情况以及对培养基的磷吸收情况 ,得到结论 :TaPT2的功能与酵母磷转运子编码基因PHO84相似 ,具有增强酵母吸收磷的作用。

Phosphorus is a major nutrient acquired by plants and phosphorus availability is considered one of the major growth limiting factors for plants in many natural ecosystems. For a better understanding of the function of the wheat phosphorus transporter gene named Ta PT2, we did Southern blot analysis and studied its function with complementation test in yeast mutant strain MB192. Southern blot indicated that Ta PT2 gene is a low copy member and has several different members in wheat genome. In the function complementation study, Ta PT2 has a similar function as PHO84 which is the phosphate transporter in yeast Saccharomyces cerevisiae . Both Ta PT2 and PHO84 are able to complement the PHO84 mutant phenotype of yeast strain MB192 which lacks the phosphate transporter activity. Therefor, this assessment presents evidence that Ta PT2 gene plays an important role in Pi acquisition.