鸟苷发酵的优化研究

STUDY ON OPTIMIZATION OF GUANOSINE FERMENTATION

以鸟苷产生菌BacillussubtilisAJ2 0 66为生产菌株 ,采用 5 0L自控发酵罐与摇瓶培养相结合的联动优化方法对鸟苷发酵进行了研究。谷氨酸钠对鸟苷发酵比较重要 ,培养基中加入 1 %的谷氨酸钠可使 5 0L罐最终产苷达 31 49g/L。次黄嘌呤 (Hx)作为前体可以直接用于鸟苷合成 ,发酵后期加入 0 2 %的Hx,可使 5 0L罐最终产苷达 33 2 4g/L。

Bacillus subtilis AJ2066 was used to yield guanosine. The optimization of guanosine fermentation was studied by means of flask coupled with 50L auto-controlled fermentor. Monosodium glutamate is important to guanosine fermentation. The yield reached 31.49g/L in 50L fermentor with the medium supplemented with 1% monosodium glutamate. Hypoxanthine (Hx) can be used to synthesize guanosine directly as precursor. The yield reached 33.24g/L in 50L fermentor by supplementing 0.2% hypoxanthine during final stage of fermentation.