全身放射损伤对皮肤伤口成纤维细胞参与组织修复能力的影响

Effects of total body irradiation injury on the participation of dermal fibroblasts in tissue repair

合并全身放射损伤的创伤 (放创复合伤 )是一种重要而有代表性的难愈性创伤 ,其难愈机制尚未完全阐明。成纤维细胞是最为重要的组织修复细胞 ,其辐射敏感性较低。为了明确放创复合伤时合并的放射损伤是否对伤口成纤维细胞有直接损伤作用 ,以及这些损伤作用对创伤愈合的影响 ,实验检测了分离、培养的放创复合伤和单纯创伤大鼠皮肤伤口成纤维细胞的增殖、凋亡及其他反映其参与组织修复能力的指标变化。结果发现 ,在去除全身因素和局部因素 ,特别是创伤局部细胞因子和细胞外基质对成纤维细胞的反馈作用后 ,放创复合伤组伤口成纤维细胞增殖力、贴壁力和粘附力均显著弱于单纯创伤组 ,而成纤维细胞的凋亡率则显著增加。这些结果表明 ,全身放射损伤对伤口成纤维细胞有直接损伤作用 ,使其参与组织修复能力显著受抑 。

Wound combined with total body irradiation (TBI) injury results in impairment of tissue repair and delayed processes of healing, so it has been considered as an important and representative model of impaired wound healing, but the mechanism is not fully clarified. Fibroblasts in wound are the most important cells participating in tissue repair, whereas its radiosenstivity is not high. To understand whether TBI injury has direct damaging effects on fibroblasts in wound, fibroblasts in wound combined with TBI injury and in wound of simple incision injury were isolated and cultured, and parameters associated with tissue repair were determined. The results showed that the abilities of proliferation, attachment and adhesion of fibroblasts isolated from wounds combined with TBI injury significantly decreased as compared with those of simple incision injury, nevertheless, apoptotic ratio of fibroblasts isolated from wounds combined with TBI injury increased significantly. These data suggest that TBI injury may cause direct damaging effects on fibroblasts in wounds, which might be one of the dominant reasons for impairment of wound healing when it is combined with TBI injury.